phospho s6 kinase thr389 Search Results


anti phospho p70s6k thr389  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc anti phospho p70s6k thr389
Anti Phospho P70s6k Thr389, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phospho p70s6k p p70s6k  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti phospho p70s6k p p70s6k
Rabbit Anti Phospho P70s6k P P70s6k, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho s6k1 thr389 mouse monoclonal antibody  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phospho s6k1 thr389 mouse monoclonal antibody
Phospho S6k1 Thr389 Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oaaf07416  (Aviva Systems)
90
Aviva Systems oaaf07416
Key resources table
Oaaf07416, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti p70 s6 kinase  (Cell Signaling Technology Inc)
91
Cell Signaling Technology Inc rabbit anti p70 s6 kinase
( A ) Western blot analysis of pY783-PLCγ, PLCγ, <t>pT389-p70-S6</t> kinase, p70-S6 kinase, pY705-STAT3 and STAT3 after treating T98G cells with 1 μM of 1α,25-dihydroxyvitamin D 3 (1α,25), 1 μM of tacalcitol (Tac) or vehicle (EtOH) for 24 h. ( B ), ( C ) and ( D ) Quantitative analysis of the levels of pY783-PLCγ, pT389-p70-S6 kinase and pY705-STAT3 inT98G cell lysates in the treatment groups as compared to vehicle. β-Actin was used as loading control for pY783-PLCγ and pT389-p70-S6. pY705-STAT3 was normalized to total STAT3. The values are presented as mean ± standard deviation of at least four independent determinations and shown as fold difference compared to ethanol-treated cells. ( E ) Analysis of STAT3 mRNA in T98G cells treated with tacalcitol (1 μM, 24h) compared to vehicle-treated cells. The graph shows the means ± standard deviation of four independent experiments. ( F ) Determination of STAT3 activation in T98G cells using a STAT3 assay kit. STAT3 activation was measured in cells cultured for 24 h in the absence (EtOH) and presence of 1α,25-dihydroxyvitamin D 3 (1α,25) or tacalcitol (Tac) in the indicated concentrations. The values are presented as mean ± standard deviation of five independent determinations. * = p < 0.05.
Rabbit Anti P70 S6 Kinase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho p70 s6 kinase thr389  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc phospho p70 s6 kinase thr389
( A ) Western blot analysis of pY783-PLCγ, PLCγ, <t>pT389-p70-S6</t> kinase, p70-S6 kinase, pY705-STAT3 and STAT3 after treating T98G cells with 1 μM of 1α,25-dihydroxyvitamin D 3 (1α,25), 1 μM of tacalcitol (Tac) or vehicle (EtOH) for 24 h. ( B ), ( C ) and ( D ) Quantitative analysis of the levels of pY783-PLCγ, pT389-p70-S6 kinase and pY705-STAT3 inT98G cell lysates in the treatment groups as compared to vehicle. β-Actin was used as loading control for pY783-PLCγ and pT389-p70-S6. pY705-STAT3 was normalized to total STAT3. The values are presented as mean ± standard deviation of at least four independent determinations and shown as fold difference compared to ethanol-treated cells. ( E ) Analysis of STAT3 mRNA in T98G cells treated with tacalcitol (1 μM, 24h) compared to vehicle-treated cells. The graph shows the means ± standard deviation of four independent experiments. ( F ) Determination of STAT3 activation in T98G cells using a STAT3 assay kit. STAT3 activation was measured in cells cultured for 24 h in the absence (EtOH) and presence of 1α,25-dihydroxyvitamin D 3 (1α,25) or tacalcitol (Tac) in the indicated concentrations. The values are presented as mean ± standard deviation of five independent determinations. * = p < 0.05.
Phospho P70 S6 Kinase Thr389, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho s6k1 t389  (Biorbyt)
90
Biorbyt phospho s6k1 t389
( A ) Western blot analysis of pY783-PLCγ, PLCγ, <t>pT389-p70-S6</t> kinase, p70-S6 kinase, pY705-STAT3 and STAT3 after treating T98G cells with 1 μM of 1α,25-dihydroxyvitamin D 3 (1α,25), 1 μM of tacalcitol (Tac) or vehicle (EtOH) for 24 h. ( B ), ( C ) and ( D ) Quantitative analysis of the levels of pY783-PLCγ, pT389-p70-S6 kinase and pY705-STAT3 inT98G cell lysates in the treatment groups as compared to vehicle. β-Actin was used as loading control for pY783-PLCγ and pT389-p70-S6. pY705-STAT3 was normalized to total STAT3. The values are presented as mean ± standard deviation of at least four independent determinations and shown as fold difference compared to ethanol-treated cells. ( E ) Analysis of STAT3 mRNA in T98G cells treated with tacalcitol (1 μM, 24h) compared to vehicle-treated cells. The graph shows the means ± standard deviation of four independent experiments. ( F ) Determination of STAT3 activation in T98G cells using a STAT3 assay kit. STAT3 activation was measured in cells cultured for 24 h in the absence (EtOH) and presence of 1α,25-dihydroxyvitamin D 3 (1α,25) or tacalcitol (Tac) in the indicated concentrations. The values are presented as mean ± standard deviation of five independent determinations. * = p < 0.05.
Phospho S6k1 T389, supplied by Biorbyt, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pathscan phospho s6kinase thr389 sandwich elisa  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc pathscan phospho s6kinase thr389 sandwich elisa
( A ) Western blot analysis of pY783-PLCγ, PLCγ, <t>pT389-p70-S6</t> kinase, p70-S6 kinase, pY705-STAT3 and STAT3 after treating T98G cells with 1 μM of 1α,25-dihydroxyvitamin D 3 (1α,25), 1 μM of tacalcitol (Tac) or vehicle (EtOH) for 24 h. ( B ), ( C ) and ( D ) Quantitative analysis of the levels of pY783-PLCγ, pT389-p70-S6 kinase and pY705-STAT3 inT98G cell lysates in the treatment groups as compared to vehicle. β-Actin was used as loading control for pY783-PLCγ and pT389-p70-S6. pY705-STAT3 was normalized to total STAT3. The values are presented as mean ± standard deviation of at least four independent determinations and shown as fold difference compared to ethanol-treated cells. ( E ) Analysis of STAT3 mRNA in T98G cells treated with tacalcitol (1 μM, 24h) compared to vehicle-treated cells. The graph shows the means ± standard deviation of four independent experiments. ( F ) Determination of STAT3 activation in T98G cells using a STAT3 assay kit. STAT3 activation was measured in cells cultured for 24 h in the absence (EtOH) and presence of 1α,25-dihydroxyvitamin D 3 (1α,25) or tacalcitol (Tac) in the indicated concentrations. The values are presented as mean ± standard deviation of five independent determinations. * = p < 0.05.
Pathscan Phospho S6kinase Thr389 Sandwich Elisa, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phospho thr389 p70 s6 kinase beta  (Biorbyt)
85
Biorbyt rabbit anti phospho thr389 p70 s6 kinase beta
( A ) Western blot analysis of pY783-PLCγ, PLCγ, <t>pT389-p70-S6</t> kinase, p70-S6 kinase, pY705-STAT3 and STAT3 after treating T98G cells with 1 μM of 1α,25-dihydroxyvitamin D 3 (1α,25), 1 μM of tacalcitol (Tac) or vehicle (EtOH) for 24 h. ( B ), ( C ) and ( D ) Quantitative analysis of the levels of pY783-PLCγ, pT389-p70-S6 kinase and pY705-STAT3 inT98G cell lysates in the treatment groups as compared to vehicle. β-Actin was used as loading control for pY783-PLCγ and pT389-p70-S6. pY705-STAT3 was normalized to total STAT3. The values are presented as mean ± standard deviation of at least four independent determinations and shown as fold difference compared to ethanol-treated cells. ( E ) Analysis of STAT3 mRNA in T98G cells treated with tacalcitol (1 μM, 24h) compared to vehicle-treated cells. The graph shows the means ± standard deviation of four independent experiments. ( F ) Determination of STAT3 activation in T98G cells using a STAT3 assay kit. STAT3 activation was measured in cells cultured for 24 h in the absence (EtOH) and presence of 1α,25-dihydroxyvitamin D 3 (1α,25) or tacalcitol (Tac) in the indicated concentrations. The values are presented as mean ± standard deviation of five independent determinations. * = p < 0.05.
Rabbit Anti Phospho Thr389 P70 S6 Kinase Beta, supplied by Biorbyt, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-phospho-p70 s6 kinase (thr389  (Merck KGaA)
90
Merck KGaA anti-phospho-p70 s6 kinase (thr389
( A ) Western blot analysis of pY783-PLCγ, PLCγ, <t>pT389-p70-S6</t> kinase, p70-S6 kinase, pY705-STAT3 and STAT3 after treating T98G cells with 1 μM of 1α,25-dihydroxyvitamin D 3 (1α,25), 1 μM of tacalcitol (Tac) or vehicle (EtOH) for 24 h. ( B ), ( C ) and ( D ) Quantitative analysis of the levels of pY783-PLCγ, pT389-p70-S6 kinase and pY705-STAT3 inT98G cell lysates in the treatment groups as compared to vehicle. β-Actin was used as loading control for pY783-PLCγ and pT389-p70-S6. pY705-STAT3 was normalized to total STAT3. The values are presented as mean ± standard deviation of at least four independent determinations and shown as fold difference compared to ethanol-treated cells. ( E ) Analysis of STAT3 mRNA in T98G cells treated with tacalcitol (1 μM, 24h) compared to vehicle-treated cells. The graph shows the means ± standard deviation of four independent experiments. ( F ) Determination of STAT3 activation in T98G cells using a STAT3 assay kit. STAT3 activation was measured in cells cultured for 24 h in the absence (EtOH) and presence of 1α,25-dihydroxyvitamin D 3 (1α,25) or tacalcitol (Tac) in the indicated concentrations. The values are presented as mean ± standard deviation of five independent determinations. * = p < 0.05.
Anti Phospho P70 S6 Kinase (Thr389, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho-p70 s6 kinase (thr389/412) antibody  (Affinity Biologicals)
90
Affinity Biologicals phospho-p70 s6 kinase (thr389/412) antibody
( A ) Western blot analysis of pY783-PLCγ, PLCγ, <t>pT389-p70-S6</t> kinase, p70-S6 kinase, pY705-STAT3 and STAT3 after treating T98G cells with 1 μM of 1α,25-dihydroxyvitamin D 3 (1α,25), 1 μM of tacalcitol (Tac) or vehicle (EtOH) for 24 h. ( B ), ( C ) and ( D ) Quantitative analysis of the levels of pY783-PLCγ, pT389-p70-S6 kinase and pY705-STAT3 inT98G cell lysates in the treatment groups as compared to vehicle. β-Actin was used as loading control for pY783-PLCγ and pT389-p70-S6. pY705-STAT3 was normalized to total STAT3. The values are presented as mean ± standard deviation of at least four independent determinations and shown as fold difference compared to ethanol-treated cells. ( E ) Analysis of STAT3 mRNA in T98G cells treated with tacalcitol (1 μM, 24h) compared to vehicle-treated cells. The graph shows the means ± standard deviation of four independent experiments. ( F ) Determination of STAT3 activation in T98G cells using a STAT3 assay kit. STAT3 activation was measured in cells cultured for 24 h in the absence (EtOH) and presence of 1α,25-dihydroxyvitamin D 3 (1α,25) or tacalcitol (Tac) in the indicated concentrations. The values are presented as mean ± standard deviation of five independent determinations. * = p < 0.05.
Phospho P70 S6 Kinase (Thr389/412) Antibody, supplied by Affinity Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho-p70 s6 kinase (thr389/412) antibody/product/Affinity Biologicals
Average 90 stars, based on 1 article reviews
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Image Search Results


Key resources table

Journal: iScience

Article Title: Mitochondrial complex I inhibitors suppress tumor growth through concomitant acidification of the intra- and extracellular environment

doi: 10.1016/j.isci.2021.103497

Figure Lengend Snippet: Key resources table

Article Snippet: The deparaffinized sections were boiled in 0.01 M buffered sodium citrate solution (pH 6.0) for 10 min and subjected to Masson's Trichrome staining (Sigma-Aldrich) overnight or immunohistochemical staining with the following antibodies for 30 min: anti-a-SMA (1:2000, ab7817, Abcam), anti-Vimentin (1:400, sc-6260, Santa Cruz Biotechnology), anti-GFP (1:500, #2955, Cell Signaling Technology), anti-Ki-67 (1:200, ab16667, Abcam), anti-Phospho-p70 S6 Kinase (Thr 389) (1:50, OAAF07416, Aviva Systems Biology, San Diego, CA), and horseradish peroxidase-linked secondary antibodies.

Techniques: Recombinant, Synthesized, Staining, Isolation, Activity Assay, Lactate Assay, Software

( A ) Western blot analysis of pY783-PLCγ, PLCγ, pT389-p70-S6 kinase, p70-S6 kinase, pY705-STAT3 and STAT3 after treating T98G cells with 1 μM of 1α,25-dihydroxyvitamin D 3 (1α,25), 1 μM of tacalcitol (Tac) or vehicle (EtOH) for 24 h. ( B ), ( C ) and ( D ) Quantitative analysis of the levels of pY783-PLCγ, pT389-p70-S6 kinase and pY705-STAT3 inT98G cell lysates in the treatment groups as compared to vehicle. β-Actin was used as loading control for pY783-PLCγ and pT389-p70-S6. pY705-STAT3 was normalized to total STAT3. The values are presented as mean ± standard deviation of at least four independent determinations and shown as fold difference compared to ethanol-treated cells. ( E ) Analysis of STAT3 mRNA in T98G cells treated with tacalcitol (1 μM, 24h) compared to vehicle-treated cells. The graph shows the means ± standard deviation of four independent experiments. ( F ) Determination of STAT3 activation in T98G cells using a STAT3 assay kit. STAT3 activation was measured in cells cultured for 24 h in the absence (EtOH) and presence of 1α,25-dihydroxyvitamin D 3 (1α,25) or tacalcitol (Tac) in the indicated concentrations. The values are presented as mean ± standard deviation of five independent determinations. * = p < 0.05.

Journal: Biochemistry and Biophysics Reports

Article Title: Effects of 1α,25-dihydroxyvitamin D 3 and tacalcitol on cell signaling and anchorage-independent growth in T98G and U251 glioblastoma cells

doi: 10.1016/j.bbrep.2022.101313

Figure Lengend Snippet: ( A ) Western blot analysis of pY783-PLCγ, PLCγ, pT389-p70-S6 kinase, p70-S6 kinase, pY705-STAT3 and STAT3 after treating T98G cells with 1 μM of 1α,25-dihydroxyvitamin D 3 (1α,25), 1 μM of tacalcitol (Tac) or vehicle (EtOH) for 24 h. ( B ), ( C ) and ( D ) Quantitative analysis of the levels of pY783-PLCγ, pT389-p70-S6 kinase and pY705-STAT3 inT98G cell lysates in the treatment groups as compared to vehicle. β-Actin was used as loading control for pY783-PLCγ and pT389-p70-S6. pY705-STAT3 was normalized to total STAT3. The values are presented as mean ± standard deviation of at least four independent determinations and shown as fold difference compared to ethanol-treated cells. ( E ) Analysis of STAT3 mRNA in T98G cells treated with tacalcitol (1 μM, 24h) compared to vehicle-treated cells. The graph shows the means ± standard deviation of four independent experiments. ( F ) Determination of STAT3 activation in T98G cells using a STAT3 assay kit. STAT3 activation was measured in cells cultured for 24 h in the absence (EtOH) and presence of 1α,25-dihydroxyvitamin D 3 (1α,25) or tacalcitol (Tac) in the indicated concentrations. The values are presented as mean ± standard deviation of five independent determinations. * = p < 0.05.

Article Snippet: The following primary antibodies were used: rabbit anti-pY783-PLCγ1 (#2821, 1:1000, Cell Signaling Technology), mouse anti-PLCγ1 (H00005335-M01, 1:1000, Novus Biologicals), mouse anti-pThr389-p70-S6 Kinase (#7053, 1:1000, Cell Signaling Technology), rabbit anti-p70-S6 Kinase (#7053, 1:1000, Cell Signaling Technology), rabbit anti-pY705-STAT3 (#9145, 1:500, Cell Signaling Technology), mouse anti-STAT3 (#9139, 1:1000, Cell Signaling Technology), rabbit anti-pS473-AKT (#4060, 1:500, Cell Signaling Technologies), mouse anti-AKT (#2920, 1:500 Cell Signaling Technologies), rabbit anti-pT202/Y204-ERK1/2 (#9101, 1:1000, Cell Signaling Technology), rabbit anti-ERK1/2 (#4695, 1:1000, Cell Signaling Technology), mouse anti-β-Actin (sc-47778, 1:1000, Santa Cruz Biotechnology), mouse anti-pT180/182-p38 (#9216, 1:1000, Cell Signaling Technology), rabbit anti-p38 (#9212, 1:1000, Cell Signaling Technology) and rabbit anti-β-Actin (ab8227, 1:1000, Abcam).

Techniques: Western Blot, Control, Standard Deviation, Activation Assay, Cell Culture